Development of r4 Gateway binary vectors (R4pGWB) enabling high-throughput promoter swapping for plant research

Tsuyoshi Nakagawa, Shinya Nakamura, Katsunori Tanaka, Makoto Kawamukai, Takamasa Suzuki, Kenzo Nakamura, Tetsuya Kimura, Sumie Ishiguro

We developed a new series of Gateway binary vectors, R4pGWBs, that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA. The structure of R4pGWBs is almost the same as the promoterless type of improved pGWBs (ImpGWBs), except that the attR1 site is replaced with attR4, which enables tripartite recombination of these vectors with promoter- and eDNA-entry clones. While ImpGWBs are suitable for promoter analysis and constitutive expression of cDNAs in higher plants, R4pGWBs have a great advantage in expressing a cDNA under the regulation of desired promoters.