{"id":812,"date":"2021-10-29T15:38:18","date_gmt":"2021-10-29T06:38:18","guid":{"rendered":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/?p=812"},"modified":"2021-10-29T17:10:31","modified_gmt":"2021-10-29T08:10:31","slug":"post-812","status":"publish","type":"post","link":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/archives\/812","title":{"rendered":"\u91cd\u306a\u3063\u305f\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306e\u6ce2\u5f62\u3092\u5206\u96e2\u3059\u308b"},"content":{"rendered":"<p>CRISPR-Cas9\u3067\u907a\u4f1d\u5b50\u306e\u7834\u58ca\u306f\u5bb9\u6613\u306b\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306e\u907a\u4f1d\u5b50\u3092\u7834\u58ca\u3059\u308b\u5834\u5408\u3001\u5f62\u8cea\u8ee2\u63db\u3092\u3057\u3066\u6700\u521d\u306b\u5f97\u3089\u308c\u305f\u7a2e\u5b50(T1)\u4e16\u4ee3\u3067\u306f\u76f8\u540c\u67d3\u8272\u4f53\u306e\u305d\u308c\u305e\u308c\u306b\u8d77\u304d\u305f\u5909\u7570\u304c\u7570\u306a\u308b\u72b6\u614b(biallelic)\u306b\u306a\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002\u3053\u306e\u5909\u7570\u3092\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306b\u3088\u3063\u3066\u8abf\u3079\u3088\u3046\u3068\u3059\u308b\u3068\u3001\u9577\u3055\u306e\u7570\u306a\u308b\u3082\u306e\u304c\u3042\u308b\u306e\u3067\u3001\u304d\u308c\u3044\u306b\u8aad\u3080\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002<br \/>\n\u3061\u3083\u3093\u3068\u8abf\u3079\u308b\u306b\u306f\u30db\u30e2\u63a5\u5408\u4f53\u3092\u3068\u308b\u304b\u3001PCR\u7523\u7269\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u3066\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u306e\u3067\u3059\u304c\u3001\u3044\u305a\u308c\u306b\u305b\u3088\u6642\u9593\u304c\u304b\u304b\u308a\u307e\u3059\u3002\u3053\u306e\u91cd\u306a\u3063\u305f\u6ce2\u5f62\u3092\u3046\u307e\u304f\u5206\u96e2\u3067\u304d\u308c\u3070\u3044\u3044\u306e\u3067\u3059\u304c\u3001\u4eba\u306e\u624b\u3067\u3084\u308b\u3068\u304b\u306a\u308a\u5927\u5909\u3067\u3059\u3002\u305d\u3053\u3067\u3001Mitsucal\u306b\u88dc\u52a9\u30d7\u30ed\u30b0\u30e9\u30e0\u3092\u5b9f\u88c5\u3057\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<h3>\u30b5\u30f3\u30d7\u30eb\u30c7\u30fc\u30bf<\/h3>\n<p>\u4ee5\u4e0b\u306e\u8aac\u660e\u624b\u9806\u306b\u4f7f\u7528\u3057\u305f\u30d5\u30a1\u30a4\u30eb\u3068\u5869\u57fa\u914d\u5217\u3067\u3059\u3002<\/p>\n<h4><a href=\"\/labo\/suzuki\/wp-content\/uploads\/2021\/10\/chromato_deconvo_sample1.ab1\">\u30b5\u30f3\u30d7\u30eb\u30d5\u30a1\u30a4\u30eb(ab1)<\/a><\/h4>\n<h4>\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3057\u305f\u7bc4\u56f2\u306e\u5869\u57fa\u914d\u5217<\/h4>\n<p><textarea style=\"width: 100%;\">AACAAGCTCTGTGTGTGGGACATGCGTCCTTATGCTCCACAAAACCGATGTGTAAAGATCTTTGAGGGGCATCAACACAACTTTGAGAAGAACCTGCTGAAATGTTCATGGTCACCTGATGGTACAAAAGTTACTGCCGGTAGCTCAGACCGTATGGTTCATATCTGGGACACAACCAGTCGGCGCACTATATATAAGCTCCCAGGCCATACCGGTTCTGTCAATGAGTGTGTTTTCCACCCAACCGAGCCTATCATTGGATCTTGCAGTAGTGATAAAAATATCTACTTGGGAGAGATCTGAATTTCAAAGCTGTGAAACCTTTGCAACTCTAAGTGGAATTGAAGTCTTTAGAATCGACGATGTGCTTTGAGACATTTTGTTTATTGAAAGAAAAAACGAAACATTGCTCTTTGGTACAAGTAATCAAGATTTGTTATCTCCTGTAATATCTTCATTACGCTCTTTATCTTCTTCTACTTTAACAGATGAGGTCATCATTTTAGTTCTCCTT<\/textarea><\/p>\n<h3>\u624b\u9806<\/h3>\n<ol>\n<li><a href=\"https:\/\/mitsucal.tsbio.info\/vl\/\">Mitsucal\u306e\u4eee\u60f3\u5b9f\u9a13\u5ba4<\/a>\u3092\u958b\u304d\u307e\u3059\u3002\u3084\u308b\u3053\u3068\u306e\u30d7\u30eb\u30c0\u30a6\u30f3\u304b\u3089\u300c\u91cd\u306a\u3063\u305f\u6ce2\u5f62\u3092\u5206\u96e2\u3059\u308b\u300d(\u3053\u306e\u540d\u79f0\u306f\u4eca\u5f8c\u5909\u66f4\u3055\u308c\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093)\u3092\u9078\u629e\u3057\u307e\u3059\u3002<img decoding=\"async\" src=\"\/labo\/suzuki\/wp-content\/uploads\/2021\/10\/812_chromato-deconvo1.png\"><\/li>\n<li>\u5869\u57fa\u914d\u5217\u3092\u53c2\u7167\u914d\u5217\u306b\u30b3\u30d4\u30fc\u3057\u307e\u3059\u3002\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u30c7\u30fc\u30bf(ab1\u30d5\u30a1\u30a4\u30eb)\u3092\u9001\u4fe1\u3057\u307e\u3059\u3002<img decoding=\"async\" src=\"\/labo\/suzuki\/wp-content\/uploads\/2021\/10\/812_chromato-deconvo2.png\"><\/li>\n<li>\u6ce2\u5f62\u30c7\u30fc\u30bf\u306e\u3046\u3061\u304d\u308c\u3044\u306b\u8aad\u3081\u3066\u3044\u308b\u3068\u3053\u308d\u3092\u898b\u3066\u307f\u307e\u3059\u3002\u307e\u305a200\u756a\u76ee\u3050\u3089\u3044\u306e\u3068\u3053\u308d\u306e\u5869\u57fa\u3092\u30af\u30ea\u30c3\u30af\u3057\u307e\u3059\u3002\u6b21\u306b\u305d\u3053\u304b\u3089\u5c11\u3057\u96e2\u308c\u305f\u3068\u3053\u308d\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u305d\u306e\u9593\u306e\u5869\u57fa\u914d\u5217\u304c\u9078\u629e\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u30d7\u30ed\u30b0\u30e9\u30e0\u3067\u306f\u5869\u57fa\u914d\u5217\u306e\u9078\u629e\u65b9\u6cd5\u304c\u7279\u6b8a\u306a\u306e\u3067\u3061\u3087\u3063\u3068\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3059\u3067\u306b\u9078\u629e\u3055\u308c\u305f\u3068\u3053\u308d\u306e\u5916\u5074\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u305d\u3053\u307e\u3067\u9078\u629e\u304c\u5ef6\u9577\u3055\u308c\u307e\u3059\u3002\u9078\u629e\u7bc4\u56f2\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u305d\u306e\u4e00\u5869\u57fa\u3060\u3051\u304c\u9078\u629e\u3055\u308c\u307e\u3059\u3002\u901a\u5e38\u306e\u30c9\u30e9\u30c3\u30b0\u3067\u306f\u9078\u629e\u3067\u304d\u306a\u3044\u306e\u3067\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<img decoding=\"async\" src=\"\/labo\/suzuki\/wp-content\/uploads\/2021\/10\/812_chromato-deconvo3.png\"><\/li>\n<li>\u5869\u57fa\u914d\u5217\u3092\u9078\u629e\u3059\u308b\u3068\u3001\u305d\u308c\u3092\u53c2\u7167\u914d\u5217\u4e2d\u306b\u63a2\u3057\u306b\u3044\u304d\u307e\u3059\u3002\u4e0b\u306e\u89e3\u6790\u7d50\u679c\u306b\u3042\u308bfound1\u304c\u898b\u3064\u304b\u3063\u305f\u914d\u5217\u3067\u3001\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306e200\u756a\u76ee\u304b\u3089214\u756a\u76ee\u306e\u5869\u57fa\u914d\u5217\u304c\u53c2\u7167\u914d\u5217\u306e153\u756a\u76ee\u304b\u3089168\u756a\u76ee\u306b\u3042\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u3068\u308a\u3048\u3042\u305a\u4eca\u306fcounter1\u306f\u7121\u8996\u3057\u3066\u304f\u3060\u3055\u3044\u3002<img decoding=\"async\" src=\"\/labo\/suzuki\/wp-content\/uploads\/2021\/10\/812_chromato-deconvo6.png\"><\/li>\n<li>\u6b21\u306b\u5869\u57fa\u914d\u5217\u304c\u91cd\u306a\u3063\u3066\u8aad\u3081\u3066\u3044\u306a\u3044\u3068\u3053\u308d\u3092\u8abf\u3079\u307e\u3059\u3002\u4f8b\u3048\u3070221\u756a\u76ee\u306fA\u306e\u6ce2\u5f62\u304c\u91cd\u306a\u3063\u3066\u3044\u307e\u3059\u304c\u3001\u3053\u3053\u306b\u70b9\u304c\u3064\u3044\u3066\u3044\u307e\u305b\u3093\u3002\u3053\u306eA\u306e\u30d4\u30fc\u30af\u3092\u30af\u30ea\u30c3\u30af\u3057\u307e\u3059\u3002\n<div><img decoding=\"async\" src=\"\/labo\/suzuki\/wp-content\/uploads\/2021\/10\/812_chromato-deconvo4.png\"><\/div>\n<\/li>\n<li>\u540c\u69d8\u306b222\u756a\u76ee\u306eA\u3001223\u756a\u76ee\u306eC\u3001224\u756a\u76ee\u306eC\u3001\u3068\u9806\u306b\u30d4\u30fc\u30af\u306f\u3042\u308b\u3051\u3069\u3001\u7121\u8996\u3055\u308c\u3066\u3044\u308b\u3082\u306e\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u3044\u304d\u307e\u3059\u3002<\/li>\n<li>229\u756a\u76ee\u306eG\u307e\u3067\u30d4\u30fc\u30af\u306e\u8ffd\u52a0\u3092\u7d42\u3048\u305f\u3089\u3001221\u304b\u3089229\u306e\u5869\u57fa\u3092\u9078\u629e\u3057\u307e\u3059(221\u756a\u76ee\u306eC\u306e\u6587\u5b57\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u3001\u6b21\u306b229\u756a\u76ee\u306eG\u3092\u30af\u30ea\u30c3\u30af\u3002\u9014\u4e2d\u3067\u5225\u306e\u7bc4\u56f2\u304c\u9078\u629e\u3055\u308c\u305f\u3089\u3001\u305d\u306e\u4e2d\u3092\u3082\u3046\u4e00\u5ea6\u30af\u30ea\u30c3\u30af)\u3002<img decoding=\"async\" src=\"\/labo\/suzuki\/wp-content\/uploads\/2021\/10\/812_chromato-deconvo5.png\"><\/li>\n<li>\u89e3\u6790\u7d50\u679c\u306efound1\u306bAACCAGTCG\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u306f\u53c2\u7167\u914d\u5217\u306e174\u756a\u76ee\u304b\u3089182\u756a\u76ee\u3068\u4e00\u81f4\u3057\u307e\u3059\u3002\u3053\u306e\u914d\u5217\u3092\u91cd\u8907\u304b\u3089\u5f15\u3044\u305f\u6b8b\u308a\u304cCCATACCGG\u3068\u3044\u3046\u914d\u5217\u3067\u3001\u3053\u308c\u304c207\u756a\u76ee\u304b\u3089215\u756a\u76ee\u3068\u4e00\u81f4\u3057\u307e\u3059\u3002\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306e214\u756a\u76ee\u306eG\u304c\u53c2\u7167\u914d\u5217\u306e168\u756a\u76ee\u3068\u4e00\u81f4\u3059\u308b\u306e\u3067\u4ee5\u4e0b\u306e\u3088\u3046\u306b\u306a\u3063\u3066\u3044\u308b\u3068\u63a8\u6e2c\u3055\u308c\u307e\u3059\u3002\n<pre>\u53c2\u7167\u914d\u5217:TTCATATCTGGGACACAACCAGTCGGCGCACTATATATAAGCTCCCAGGCCATACCGGTTCTGTCAATGAGTGTG\n\u30a2\u30ea\u30eb1: TTCATATCTGGxAxACAACCAGTCG\n\u30a2\u30ea\u30eb2: TTCATATCTGGxAxAC------(30\u5869\u57fa\u3050\u3089\u3044\u306e\u6b20\u5931)-------CCATACCGG\n<\/pre>\n<\/li>\n<\/ol>\n<h3>\u6ce8\u610f<\/h3>\n<ul>\n<li>\u4eca\u306e\u3068\u3053\u308d\u76f8\u88dc\u9396\u306e\u691c\u7d22\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u65b9\u5411\u306b\u5408\u308f\u305b\u3066\u53c2\u7167\u914d\u5217\u3092\u7528\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u30ae\u30e3\u30c3\u30d7\u3092\u8003\u616e\u3057\u305f\u691c\u7d22\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u4e0a\u8a18\u306e\u4f8b\u3067\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306e215\u756a\u3042\u305f\u308a\u306e\u5869\u57fa\u3092\u542b\u3093\u3067\u691c\u7d22\u3092\u304b\u3051\u308b\u3068\u53c2\u7167\u914d\u5217\u3092\u898b\u3064\u3051\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002CRISPR-Cas9\u306e\u5207\u65ad\u4f4d\u7f6e\u4ed8\u8fd1\u304b\u3089\u5c11\u3057\u96e2\u308c\u305f\u3068\u3053\u308d\u3067\u4e00\u81f4\u70b9\u3092\u63a2\u3057\u3066\u3001\u305d\u3053\u304b\u3089\u5f90\u3005\u306b\u4e00\u81f4\u3057\u306a\u304f\u306a\u308b\u3068\u3053\u308d\u307e\u3067\u5ef6\u3070\u3057\u3066\u3044\u304f\u3068\u3088\u3044\u3067\u3057\u3087\u3046\u3002<\/li>\n<li>\u633f\u5165\u914d\u5217\u3092\u898b\u3064\u3051\u308b\u306e\u3082\u96e3\u3057\u3044\u3067\u3059\u3002\u633f\u5165\u306f\u305f\u3076\u3093\u305d\u308c\u307b\u3069\u9577\u304f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3089\u300150\u5869\u57fa\u3050\u3089\u3044\u4e0b\u6d41\u304b\u3089\u691c\u7d22\u3092\u958b\u59cb\u3057\u3066\u3001\u53c2\u7167\u914d\u5217\u3068\u4e00\u81f4\u3057\u306a\u304f\u306a\u308b\u5834\u6240\u307e\u3067\u5ef6\u3070\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<h3>\u4f3c\u305f\u6a5f\u80fd\u306e\u30d7\u30ed\u30b0\u30e9\u30e0<\/h3>\n<p>\u3048\u3048\u3001\u3082\u3061\u308d\u3093Mitsucal\u306e\u6a5f\u80fd\u62e1\u5f35\u306e\u76ee\u9014\u304c\u3064\u3044\u3066\u304b\u3089\u898b\u3064\u3051\u3066\u3057\u307e\u3044\u307e\u3057\u305f(\u8eca\u8f2a\u306e\u518d\u767a\u660e\u4e07\u6b73)\u3002<\/p>\n<h4><a href=\"http:\/\/yosttools.genetics.utah.edu\/PolyPeakParser\/\">POLY PEAK PARSER<\/a><\/h4>\n<p>\u91ce\u751f\u578b\u3068\u6b20\u5931\u306e\u30d8\u30c6\u30ed\u63a5\u5408\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u304c\u89e3\u6790\u3067\u304d\u308b\u3063\u307d\u3044\u3002<\/p>\n<h4><a href=\"http:\/\/crispid.gbiomed.kuleuven.be\/\">CRISP-ID<\/a><\/h4>\n<p>\u4eca\u306f\u52d5\u3044\u3066\u3044\u306a\u3055\u305d\u3046\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CRISPR-Cas9\u3067\u907a\u4f1d\u5b50\u306e\u7834\u58ca\u306f\u5bb9\u6613\u306b\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306e\u907a\u4f1d\u5b50\u3092\u7834\u58ca\u3059\u308b\u5834\u5408\u3001\u5f62\u8cea\u8ee2\u63db\u3092\u3057\u3066\u6700\u521d\u306b\u5f97\u3089\u308c\u305f\u7a2e\u5b50(T1)\u4e16\u4ee3\u3067\u306f\u76f8\u540c\u67d3\u8272\u4f53\u306e\u305d\u308c\u305e\u308c\u306b\u8d77\u304d\u305f\u5909\u7570\u304c\u7570\u306a\u308b\u72b6\u614b(biallelic) [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,7,14],"tags":[30],"_links":{"self":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/812"}],"collection":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/comments?post=812"}],"version-history":[{"count":25,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/812\/revisions"}],"predecessor-version":[{"id":849,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/812\/revisions\/849"}],"wp:attachment":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/media?parent=812"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/categories?post=812"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/tags?post=812"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}