Plant & Cell Physilogy<\/a><\/p>\nSummary<\/h3>\n
Many eukaryotes possess two types of spliceosomes: the U2-dependent and U12-dependent spliceosomes. The U2dependent spliceosome processes >99% of all introns, whereas the U12-dependent spliceosome acts on only \u223c0.3% of introns, one-third of which start with AT and end with AC, with the remainder having GT\u2013AG termini. How the U12-dependent spliceosome splices two types of introns with different terminal sequences remains poorly understood.<\/p>\n
Human centrosomal AT–AC splicing factor (CENATAC) is a subunit of the U12-dependent spliceosome that is particularly required for the splicing of the AT–AC subtype. The Arabidopsis genome contains a single homolog, TITAN-LIKE<\/i> (TTL<\/i>), but its function in splicing remains unknown. <\/p>\n
Here, we generated ttl<\/i> mutants and isolated two viable alleles, of which we analyzed one, designated ttl-142<\/i>, to investigate TTL\u2019s function in splicing. ttl-142<\/i> carries a 42-nucleotide deletion that removes 14 amino acid residues from the predicted protein, and homozygous mutants exhibit morphological abnormalities. Most U12-dependent introns were less efficiently spliced in ttl-142<\/i> than in the wild type, with the splicing of AT–AC introns particularly suppressed. <\/p>\n
Splicing suppression in ttl-142<\/i> was more extensive than in a drol1<\/i> (defective repression of the OLE3:LUC1<\/i>) mutant, which carries a mutation in a gene specifically required for AT–AC intron splicing. Conversely, fewer genes showed altered expression levels in ttl-142<\/i> than in drol1, and most differentially expressed genes differed between the two mutants. <\/p>\n
These results suggest that the phenotypes of ttl-142<\/i> and drol1<\/i> mutants may reflect the impairment of distinct spliceosomal functions.<\/p>\n\u8981\u7d04<\/h4>\n
\u591a\u304f\u306e\u771f\u6838\u751f\u7269\u306f\u3001U2\u4f9d\u5b58\u578b\u30b9\u30d7\u30e9\u30a4\u30bd\u30bd\u30fc\u30e0\u3068U12\u4f9d\u5b58\u578b\u30b9\u30d7\u30e9\u30a4\u30bd\u30bd\u30fc\u30e0\u3068\u3044\u3046\u4e8c\u7a2e\u985e\u306e\u30b9\u30d7\u30e9\u30a4\u30bd\u30bd\u30fc\u30e0\u3092\u6301\u3064\u3002U2\u4f9d\u5b58\u578b\u30b9\u30d7\u30e9\u30a4\u30bd\u30bd\u30fc\u30e0\u306f99\uff05\u4ee5\u4e0a\u306e\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u3092\u62c5\u3046\u306e\u306b\u5bfe\u3057\u3001U12\u4f9d\u5b58\u578b\u30b9\u30d7\u30e9\u30a4\u30bd\u30bd\u30fc\u30e0\u306f\u308f\u305a\u304b0.3\uff05\u306e\u30a4\u30f3\u30c8\u30ed\u30f3\u3092\u6a19\u7684\u3068\u3059\u308b\u3002U12\u578b\u30a4\u30f3\u30c8\u30ed\u30f3\u306e\u3046\u30613\u5206\u306e1\u306f\u305d\u306e\u5869\u57fa\u914d\u5217\u304cAT\u3067\u59cb\u307e\u308aAC\u3067\u7d42\u308f\u308bAT–AC\u578b\u3067\u3042\u308a\u3001\u6b8b\u308a\u306fGT–AG\u672b\u7aef\u3092\u3082\u3064\u3002U12\u4f9d\u5b58\u578b\u30b9\u30d7\u30e9\u30a4\u30bd\u30bd\u30fc\u30e0\u304c\u3001\u672b\u7aef\u914d\u5217\u306e\u7570\u306a\u308b\u4e8c\u7a2e\u985e\u306e\u30a4\u30f3\u30c8\u30ed\u30f3\u3092\u3069\u306e\u3088\u3046\u306b\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u3057\u3066\u3044\u308b\u306e\u304b\u306f\u3001\u3088\u304f\u5206\u304b\u3063\u3066\u3044\u306a\u3044\u3002<\/p>\n
\u30d2\u30c8\u306ecentrosomal AT\u2013AC splicing factor (CENATAC)\u306fU12\u4f9d\u5b58\u578b\u30b9\u30d7\u30e9\u30a4\u30bd\u30bd\u30fc\u30e0\u306e\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u3067\u3042\u308a\u3001\u7279\u306bAT–AC\u578b\u30a4\u30f3\u30c8\u30ed\u30f3\u306e\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u306b\u5fc5\u9808\u3067\u3042\u308b\u3002\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306e\u30b2\u30ce\u30e0\u306b\u306f\u305d\u306e\u30db\u30e2\u30ed\u30b0\u304c\u4e00\u3064\u5b58\u5728\u3057\u3001TITAN-LIKE<\/i> (TTL<\/i>)\u3068\u547c\u3070\u308c\u308b\u304c\u3001\u305d\u306e\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u306b\u304a\u3051\u308b\u6a5f\u80fd\u306f\u4e0d\u660e\u3067\u3042\u3063\u305f\u3002<\/p>\n
\u672c\u7814\u7a76\u3067\u306fttl<\/i>\u5909\u7570\u4f53\u3092\u4f5c\u51fa\u3057\u3001\u305d\u306e\u3046\u3061\u306e\u751f\u5b58\u53ef\u80fd\u306a\u30a2\u30ea\u30eb(ttl-142<\/i>)\u3092\u89e3\u6790\u3057\u3001TTL\u306e\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u306b\u304a\u3051\u308b\u6a5f\u80fd\u3092\u8abf\u3079\u305f\u3002ttl-142<\/i>\u306b\u306f42\u5869\u57fa\u306e\u6b20\u5931\u304c\u3042\u308a\u300114\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u3092\u6b20\u5931\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u767a\u73fe\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3001\u305d\u306e\u30db\u30e2\u63a5\u5408\u4f53\u306f\u5f62\u614b\u7570\u5e38\u3092\u793a\u3057\u305f\u3002\u591a\u304f\u306eU12\u4f9d\u5b58\u578b\u30a4\u30f3\u30c8\u30ed\u30f3\u306f\u91ce\u751f\u578b\u306b\u6bd4\u3079\u3066 ttl-142<\/i>\u3067\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u52b9\u7387\u304c\u4f4e\u4e0b\u3057\u3001\u3068\u304f\u306bAT–AC\u578b\u30a4\u30f3\u30c8\u30ed\u30f3\u306e\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u304c\u5f37\u304f\u6291\u5236\u3055\u308c\u3066\u3044\u305f\u3002<\/p>\n
\u3053\u306e\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u6291\u5236\u306f\u3001AT–AC\u578b\u30a4\u30f3\u30c8\u30ed\u30f3\u306e\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u306b\u7279\u7570\u7684\u306b\u5fc5\u8981\u306a\u907a\u4f1d\u5b50\u306b\u5909\u7570\u3092\u3082\u3064 drol1<\/i>\uff08defective repression of the OLE3:LUC1<\/i>\uff09\u5909\u7570\u4f53\u3088\u308a\u3082\u5e83\u7bc4\u3067\u3042\u3063\u305f\u3002\u4e00\u65b9\u3067\u3001ttl-142<\/i>\u3067\u306f drol1<\/i>\u3088\u308a\u3082\u767a\u73fe\u91cf\u304c\u5909\u52d5\u3059\u308b\u907a\u4f1d\u5b50\u304c\u5c11\u306a\u304f\u3001\u307e\u305f\u767a\u73fe\u91cf\u306e\u5909\u5316\u3057\u305f\u907a\u4f1d\u5b50\u306e\u591a\u304f\u306f\u4e21\u5909\u7570\u4f53\u9593\u3067\u4e00\u81f4\u3057\u306a\u304b\u3063\u305f\u3002<\/p>\n
\u3053\u308c\u3089\u306e\u7d50\u679c\u306f\u3001ttl-142<\/i>\u3068drol1<\/i>\u306e\u8868\u73fe\u578b\u304c\u3001\u30b9\u30d7\u30e9\u30a4\u30bd\u30bd\u30fc\u30e0\u5185\u306e\u7570\u306a\u308b\u6a5f\u80fd\u306e\u969c\u5bb3\u3092\u53cd\u6620\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p>\n
<\/p>\n","protected":false},"excerpt":{"rendered":"
\u5f53\u7814\u7a76\u5ba4\u306e\u8ad6\u6587 Arabidopsis TITAN-LIKE is required for U12-type intron splicing, especially of AT\u2013AC subtypes \u304c Plant […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/2644"}],"collection":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/comments?post=2644"}],"version-history":[{"count":2,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/2644\/revisions"}],"predecessor-version":[{"id":2646,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/2644\/revisions\/2646"}],"wp:attachment":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/media?parent=2644"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/categories?post=2644"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/tags?post=2644"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}