{"id":2476,"date":"2025-09-01T00:00:00","date_gmt":"2025-08-31T15:00:00","guid":{"rendered":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/archives\/2476"},"modified":"2025-09-01T00:00:00","modified_gmt":"2025-08-31T15:00:00","slug":"drol1-dib1-determines-u5-snrnp-specificity-for-intron-terminal-dinucleotide-in-arabidopsis","status":"publish","type":"post","link":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/archives\/2476","title":{"rendered":"DROL1\/DIB1 determines U5 snRNP specificity for intron terminal dinucleotide in Arabidopsis."},"content":{"rendered":"<p class='author'>Takamasa Suzuki, Tomoko Niwa, Ayami Furuta, Daisuke Aramaki, Kokone Toyama, Mizuho Ito, Rio Kikuchi, Sanetaka Ishikawa, Takeki Ohsumi, Tatsuki Inonue, Yoshiaki Shiotani, Yuma Ito, Yuriko Inami<\/p>\n<p class='abstract en'>Most introns contain GT-AG terminal dinucleotides; although some eukaryotes have introns with AT-AC termini whose splicing is impaired in the Arabidopsis defective repression of OLE3::LUC 1 (drol1) mutant. We identified seven drol1 suppressors across four loci, all encoding subunits of the U5 snRNP. Although AT-AC splicing was partially restored in these suppressors, their phenotypes were almost completely rescued. Artificial introns with either GT-AG or AT-AC termini showed a splicing preference for GT-AG termini in drol1 and its suppressors. These results suggest that AT-AC introns are spliced by a GT-AG specific spliceosome in the suppressors, with DROL1 influencing U5 snRNP specificity at the 5&#8242; splice site. We propose that mRNAs retaining unspliced AT-AC introns impair translation and produce nuclear signals that contribute to the complex phenotypes seen in drol1.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Takamasa Suzuki, Tomoko Niwa, Ayami Furuta, Daisuke Aramaki, Kokone Toyama, Mizuho Ito, Rio Kikuchi, Sanetaka  [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"_links":{"self":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/2476"}],"collection":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/comments?post=2476"}],"version-history":[{"count":0,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/2476\/revisions"}],"wp:attachment":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/media?parent=2476"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/categories?post=2476"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/tags?post=2476"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}