{"id":2160,"date":"2024-03-13T00:00:00","date_gmt":"2024-03-12T15:00:00","guid":{"rendered":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/archives\/2160"},"modified":"2024-03-13T00:00:00","modified_gmt":"2024-03-12T15:00:00","slug":"post-0","status":"publish","type":"post","link":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/archives\/2160","title":{"rendered":"Group C <scp>MAP<\/scp> kinases phosphorylate <scp>MBD10<\/scp> to regulate <scp>ABA<\/scp>\u2010induced leaf senescence in Arabidopsis"},"content":{"rendered":"<p class='author'>Yangdan Li, Yoshiaki Kamiyama, Fuko Minegishi, Yuki Tamura, Kota Yamashita, Sotaro Katagiri, Hinano Takase, Masahiko Otani, Ryo Tojo, Gabrielle E. Rupp, Takamasa Suzuki, Naoto Kawakami, Scott C. Peck, Taishi Umezawa<\/p>\n<p class='abstract en'>SUMMARY<\/p>\n<p>Abscisic acid (ABA) is a phytohormone that promotes leaf senescence in response to environmental stress. We previously identified methyl CpG\u2010binding domain 10 (MBD10) as a phosphoprotein that becomes differentially phosphorylated after ABA treatment in Arabidopsis. ABA\u2010induced leaf senescence was delayed in mbd10 knockout plants but accelerated in MBD10\u2010overexpressing plants, suggesting that MBD10 positively regulates ABA\u2010induced leaf senescence. ABA\u2010induced phosphorylation of MBD10 occurs in planta on Thr\u201089, and our results demonstrated that Thr\u201089 phosphorylation is essential for MBD10&#8217;s function in leaf senescence. The in vivo phosphorylation of Thr\u201089 in MBD10 was significantly downregulated in a quadruple mutant of group C MAPKs (mpk1\/2\/7\/14), and group C MAPKs directly phosphorylated MBD10 in vitro. Furthermore, mpk1\/2\/7\/14 showed a similar phenotype as seen in mbd10 for ABA\u2010induced leaf senescence, suggesting that group C MAPKs are the cognate kinases of MBD10 for Thr\u201089. Because group C MAPKs have been reported to function downstream of SnRK2s, our results indicate that group C MAPKs and MBD10 constitute a regulatory pathway for ABA\u2010induced leaf senescence.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Yangdan Li, Yoshiaki Kamiyama, Fuko Minegishi, Yuki Tamura, Kota Yamashita, Sotaro Katagiri, Hinano Takase, Ma [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"_links":{"self":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/2160"}],"collection":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/comments?post=2160"}],"version-history":[{"count":0,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/2160\/revisions"}],"wp:attachment":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/media?parent=2160"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/categories?post=2160"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/tags?post=2160"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}