{"id":1217,"date":"2021-05-25T00:00:00","date_gmt":"2021-05-24T15:00:00","guid":{"rendered":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/archives\/1217"},"modified":"2021-05-25T00:00:00","modified_gmt":"2021-05-24T15:00:00","slug":"transcriptomic-analysis-of-resistant-and-susceptible-responses-in-a-new-model-root-knot-nematode-infection-system-using-solanum-torvum-and-meloidogyne-arenaria","status":"publish","type":"post","link":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/archives\/1217","title":{"rendered":"Transcriptomic Analysis of Resistant and Susceptible Responses in a New Model Root-Knot Nematode Infection System Using Solanum torvum and Meloidogyne arenaria"},"content":{"rendered":"<p class='author'>Kazuki Sato, Taketo Uehara, Julia Holbein, Yuko Sasaki-Sekimoto, Pamela Gan, Takahiro Bino, Katsushi Yamaguchi, Yasunori Ichihashi, Noriko Maki, Shuji Shigenobu, Hiroyuki Ohta, Rochus B. Franke, Shahid Siddique, Florian M. W. Grundler, Takamasa Suzuki, Yasuhiro Kadota, Ken Shirasu<\/p>\n<p class='abstract en'>Root-knot nematodes (RKNs) are among the most devastating pests in agriculture. <italic>Solanum torvum<\/italic> Sw. (Turkey berry) has been used as a rootstock for eggplant (aubergine) cultivation because of its resistance to RKNs, including <italic>Meloidogyne incognita<\/italic> and <italic>M. arenaria<\/italic>. We previously found that a pathotype of <italic>M. arenaria<\/italic>, A2-J, is able to infect and propagate in <italic>S. torvum. In vitro<\/italic> infection assays showed that <italic>S. torvum<\/italic> induced the accumulation of brown pigments during avirulent pathotype A2-O infection, but not during virulent A2-J infection. This experimental system is advantageous because resistant and susceptible responses can be distinguished within a few days, and because a single plant genome can yield information about both resistant and susceptible responses. Comparative RNA-sequencing analysis of <italic>S. torvum<\/italic> inoculated with A2-J and A2-O at early stages of infection was used to parse the specific resistance and susceptible responses. Infection with A2-J did not induce statistically significant changes in gene expression within one day post-inoculation (DPI), but afterward, A2-J specifically induced the expression of chalcone synthase, spermidine synthase, and genes related to cell wall modification and transmembrane transport. Infection with A2-O rapidly induced the expression of genes encoding class III peroxidases, sesquiterpene synthases, and fatty acid desaturases at 1 DPI, followed by genes involved in defense, hormone signaling, and the biosynthesis of lignin at 3 DPI. Both isolates induced the expression of suberin biosynthetic genes, which may be triggered by wounding during nematode infection. Histochemical analysis revealed that A2-O, but not A2-J, induced lignin accumulation at the root tip, suggesting that physical reinforcement of cell walls with lignin is an important defense response against nematodes. The <italic>S. torvum<\/italic>-RKN system can provide a molecular basis for understanding plant-nematode interactions.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Kazuki Sato, Taketo Uehara, Julia Holbein, Yuko Sasaki-Sekimoto, Pamela Gan, Takahiro Bino, Katsushi Yamaguchi [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"_links":{"self":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/1217"}],"collection":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/comments?post=1217"}],"version-history":[{"count":0,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/1217\/revisions"}],"wp:attachment":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/media?parent=1217"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/categories?post=1217"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/tags?post=1217"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}