{"id":1215,"date":"2021-08-19T00:00:00","date_gmt":"2021-08-18T15:00:00","guid":{"rendered":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/archives\/1215"},"modified":"2021-08-19T00:00:00","modified_gmt":"2021-08-18T15:00:00","slug":"dna-methyltransferase-chromomethylase3-prevents-onsen-transposon-silencing-under-heat-stress","status":"publish","type":"post","link":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/archives\/1215","title":{"rendered":"DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress"},"content":{"rendered":"<p class='author'>Kosuke Nozawa, Jiani Chen, Jianjun Jiang, Sarah M. Leichter, Masataka Yamada, Takamasa Suzuki, Fengquan Liu, Hidetaka Ito, Xuehua Zhong<\/p>\n<p class='abstract en'>DNA methylation plays crucial roles in transposon silencing and genome integrity. CHROMOMETHYLASE3 (CMT3) is a plant-specific DNA methyltransferase responsible for catalyzing DNA methylation at the CHG (H = A, T, C) context. Here, we identified a positive role of CMT3 in heat-induced activation of retrotransposon <italic>ONSEN<\/italic>. We found that the full transcription of <italic>ONSEN<\/italic> under heat stress requires CMT3. Interestingly, loss-of-function CMT3 mutation led to increased CHH methylation at <italic>ONSEN<\/italic>. The CHH methylation is mediated by CMT2, as evidenced by greatly reduced CHH methylation in <italic>cmt2<\/italic> and <italic>cmt2 cmt3<\/italic> mutants coupled with increased <italic>ONSEN<\/italic> transcription. Furthermore, we found more CMT2 binding at <italic>ONSEN<\/italic> chromatin in <italic>cmt3<\/italic> compared to wild-type accompanied with an ectopic accumulation of H3K9me2 under heat stress, suggesting a collaborative role of H3K9me2 and CHH methylation in preventing heat-induced <italic>ONSEN<\/italic> activation. In summary, this study identifies a non-canonical role of CMT3 in preventing transposon silencing and provides new insights into how DNA methyltransferases regulate transcription under stress conditions.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Kosuke Nozawa, Jiani Chen, Jianjun Jiang, Sarah M. Leichter, Masataka Yamada, Takamasa Suzuki, Fengquan Liu, H [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"_links":{"self":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/1215"}],"collection":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/comments?post=1215"}],"version-history":[{"count":0,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/posts\/1215\/revisions"}],"wp:attachment":[{"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/media?parent=1215"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/categories?post=1215"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biochemistry.isc.chubu.ac.jp\/labo\/suzuki\/wp-json\/wp\/v2\/tags?post=1215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}